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Clinical Chemistry 17: 1171-1175, 1971;
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Clinical Chemistry, Vol 17, 1171-1175, Copyright © 1971 by the American Association for Clinical Chemistry

Evaluation of an Automatic Calcium Titrator

Roy L. Alexander Jr. 1

1 Department of Pathology, St. Louis University Hospitals, 1325 S. Grand Blvd., St. Louis, Mo. 63104.

An automatic calcium titrator for determining total serum calcium concentration has been evaluated. The instrument incorporates a motorized buret, a fluorometer, and a digital readout that is responsive to the quenching of the fluorescent calcium—calcein complex by the chelating agent, ethyleneglycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid. Analyses made with the calcium titrator were compared with those made with the SMA 12/60 and atomic absorption spectrophotometry. Good correlation was obtained in each instance. Slight hemolysis and bilirubin concentrations near normal did not affect the results; however, increased concentrations of these substances resulted in decreased values. The precision of analysis depends on the technique used in pipetting the sample. A single analysis of 0.1 ml of serum can be completed in 1 to 2 min. With careful analytical technique, precision is good (CV, 0.72%).


Key Words: atomic absorption spectrophotometry • AutoAnalyzer • effect of bilirubin, hemoglobin • calcein fluorometry • EGTA

Submitted on August 2, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.