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Clinical Chemistry 17: 1186-1190, 1971;
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Clinical Chemistry, Vol 17, 1186-1190, Copyright © 1971 by the American Association for Clinical Chemistry

A Facilitated Fractionation of Stable Urinary Estrogens before Their Automated Fluorometric Assay

Herbert S. Strickler 1, Judith M. Petty 1, and Patricia J. Stanchak 1

1 Section of Research in Endocrinology and Metabolism, William H. Singer Memorial Research Institute of the Allegheny General Hospital, Pittsburgh, Pa. 15212.

Urine samples to be assayed for estrone, estradiol-17beta, and estriol are processed as usual: hydrolysis, ether extraction, and saponification, as well as partition between toluene and alkali. The samples are purified further, fractionated, and assayed by connecting columns containing Sephadex LH-20 to the sample line of an automated system for fluorometric assay. With this procedure, a sample can be fractionated and assayed every 45 min.


Key Words: Sephadex chromatography • acid hydrolysis of estrogen conjugates • Celite • AutoAnalyzer • total estrogens in pregnancy urine • diagnostic aid

Submitted on June 8, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.