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Clinical Chemistry 17: 1196-1199, 1971;
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Clinical Chemistry, Vol 17, 1196-1199, Copyright © 1971 by the American Association for Clinical Chemistry

Application of Fe(II)-5-Pyridyl Benzodiazepin-2-ones to the Determination of Plasma or Serum Glucose

Bernard Klein 1 and Lois B. Lucas 1

1 Department of Diagnostic Research, Hoffmann— La Roche Inc., Nutley, N.J. 07110.

A new coupled redox procedure has been developed for the determination of glucose in 4 µl of plasma or serum. The photometric endpoint is formation of the violet-blue Fe2+-5-pyridyl benzodiazepin-2-one chelate, for which the absorbance at 580 nm is linear with glucose concentration from 25 to 300 mg/100 ml. Results obtained by the present technique and those obtained by the o-toluidine method and a glucose oxidase method agree well. The mean recovery of glucose added to plasma is 96.6% (range: 92.1-99.6%).


Key Words: coupled redox reaction • ferricyanide • iron ligand chelate

Submitted on July 15, 1971
Accepted on August 26, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.