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Clinical Chemistry 17: 1200-1201, 1971;
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Clinical Chemistry, Vol 17, 1200-1201, Copyright © 1971 by the American Association for Clinical Chemistry

Simplified Benzophenone Procedure for Determination of Diphenylhydantoin in Plasma

Wesley A. Dill 1, Lucy Chucot 1, Tsun Chang 1, and Anthony J. Glazko 1

1 Department of Pharmacology, Division of Medical and Scientific Affairs, Parke, Davis & Co., Ann Arbor, Mich. 48106.

Diphenylhydantoin (DPH) is extracted from plasma with ethylene dichloride, returned to alkali, and oxidized with permanganate in glass-stoppered test tubes containing a layer of iso-octane. The absorbance of the oxidation product (benzophenone) in the iso-octane layer is measured spectrophotometrically. This procedure eliminates interference by chloroform that is encountered in other methods; highly specialized reflux condensers or distillation equipment is not needed; and the cost of solvents is decreased. The procedure is highly specific; it detects less than 1 µg of DPH per milliliter of plasma, and it is suitable for routine clinical laboratory use.


Key Words: 1,2-dichloroethane as extractant

Submitted on July 24, 1971
Accepted on August 26, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.