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Clinical Chemistry 17: 103-107, 1971;
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Clinical Chemistry, Vol 17, 103-107, Copyright © 1971 by the American Association for Clinical Chemistry

Modified Manual or Automated o-Toluidine System for Determining Glucose in Serum, with an Improved Aqueous Reagent

Hugh Y. Yee 1, Edward S. Jenest 1, and Fred R. Bowles 1

1 Department of Pathology, Hutzel Hospital, 432 E. Hancock St., Detroit, Mich. 48201.

A manual and automated method has been developed in which a new "aqueous" o-toluidine reagent is used that is compatible with standard tubing throughout the system. Citric acid, boric acid, and thiourea are incorporated, to increase absorbance readings. The sampling rate is 50/h, and the sample volume is 0.12 ml. Measurements may be made at either 630 or 385 nm.


Key Words: citric acid, boric acid, and thiourea additives

Submitted on August 17, 1970
Accepted on November 6, 1970






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Copyright © 1971 by the American Association for Clinical Chemistry.