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Clinical Chemistry, Vol 17, 108-110, Copyright © 1971 by the American Association for Clinical Chemistry
1 Departments of Pediatrics and Laboratory Medicine,
University of Minnesota College of Medicine, Minneapolis,
Minn. 55455.
A method is presented for determining erythrocyte sodium and potassium concentration. Heparinized whole blood is separated into cellular and plasma fractions in large-bore capillary tubes. An aliquot of the erythrocyte fraction is then pipetted directly into lithium chloride diluent and the electrolytes determined flame photometrically. Plasma trapping is measured with 14C-inulin and corrections calculated for trapped sodium and potassium. This technique is rapid, simple, and can be performed as a routine procedure by most clinical laboratories.
Accepted on November 3, 1970
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