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Clinical Chemistry 17: 111-114, 1971;
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Clinical Chemistry, Vol 17, 111-114, Copyright © 1971 by the American Association for Clinical Chemistry

Electrophoretic Separation of Serum Lipoproteins in Polyacrylamide Gel

Christopher S. Frings 1, Lowell B. Foster 1, and Patricia S. Cohen 1

1 Medical Laboratory Associates, 1025 S. 18th St., Birmingham, Ala. 35205.

A method is described for electrophoresing lipoproteins in polyacrylamide gels, in which separations depend both on electrophoretic mobility and molecular size. The sample is prestained with Sudan Black B in a sample gel and then resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel, and separating gel. This method was compared with the paper electrophoretic method of Lees and Hatch [J. Lab. Clin. Med. 61, 518(1963)] and was found to be equally useful in phenotyping lipoproteinemias, with two distinct improvements over paper electrophoretic methods: lipoproteins are more quickly and discretely separated.

Submitted on October 8, 1970
Accepted on November 23, 1970




The following articles in journals at HighWire Press have cited this article:


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 J. Lipid Res.Home page
Y. Hirowatari, H. Yoshida, H. Kurosawa, K.-i. Doumitu, and N. Tada
Measurement of cholesterol of major serum lipoprotein classes by anion-exchange HPLC with perchlorate ion-containing eluent
J. Lipid Res., July 1, 2003; 44(7): 1404 - 1412.
[Abstract] [Full Text] [PDF]


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Copyright © 1971 by the American Association for Clinical Chemistry.