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Clinical Chemistry 17: 72-77, 1971;
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Clinical Chemistry, Vol 17, 72-77, Copyright © 1971 by the American Association for Clinical Chemistry

Separation and Identification of Urinary Constituents by Use of Multiple-Analytical Techniques

J. E. Mrochek 1, W. C. Butts 1, W. T. Rainey Jr. 1, and C. A. Burtis 1

1 Oak Ridge, National Laboratory, Oak Ridge, Tenn. 37830 (operated for the U.S. Atomic Energy Commission by Union Carbide Corp.).

High-pressure anion-exchange chromatography with gradient elution has been used to resolve ultraviolet-absorbing constituents from normal and pathologic specimens of human urine. We have identified 50 of these constituents by using multiple-analytical techniques. Ultraviolet spectral maxima, gas chromatographic methylene unit (MU) retention values (for trimethylsilyl derivatives), and the major mass spectral peaks are reported for each of the identified compounds.


Key Words: anion-exchange chromatography • methylene unit retention values • uv spectra • mass spectrometry • gas-liquid chromatography

Submitted on October 2, 1970
Accepted on October 26, 1970




The following articles in journals at HighWire Press have cited this article:


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 ScienceHome page
C. D. Scott
High-Pressure Ion Exchange Chromatography
Science, October 18, 1974; 186(4160): 226 - 233.
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Copyright © 1971 by the American Association for Clinical Chemistry.