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Clinical Chemistry, Vol 17, 145-147, Copyright © 1971 by the American Association for Clinical Chemistry
1 Laboratorio di Biochimica della Simes S.p.A., Via
Bellerio 41, 20161 Milano, Italy.
A sensitive (5 to 100 µg) and reproducible (±3.54% SD) method is described for determining monoglycerides, diglycerides, triglycerides, and free fatty acids. After these lipid classes are separated from extracts of plasma or tissues by a single thin-layer chromatographic development, they are eluted into concentrated H2SO4 at 200°C. The resulting color is stable for more than six days.
Submitted on October 19, 1970
Accepted on October 28, 1970
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