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Clinical Chemistry 17: 158-160, 1971;
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Clinical Chemistry, Vol 17, 158-160, Copyright © 1971 by the American Association for Clinical Chemistry

A Simplified Alkaline Phosphotungstate Assay for Uric Acid in Serum

James J. Carroll 1, Holly Coburn 1, Roberta Douglass 1, and Arthur L. Babson 1

1 Diagnostics Research Department, Warner-Lambert Research Institute, 170 Tabor Rd., Morris Plains, N.J. 07950.

An alkaline phosphotungstate procedure was developed in which the phosphotungstic acid reagent is used both as protein-precipitating and oxidizing reagent. The procedure requires only a 0.2-ml sample and fewer reagents and manipulations than similar procedures, but yields results equivalent to those obtained by Henry’s alkaline phosphotungstate procedure. Recovery, precision, and linearity were good over a wide range of uric acid concentrations. Standardization was easy with use of aqueous albumin standards or of commercial calibration reference sera.


Key Words: nonspecific chromogens • ascorbic acid • commercial reference sera

Submitted on October 30, 1970
Accepted on December 24, 1970







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Copyright © 1971 by the American Association for Clinical Chemistry.