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Clinical Chemistry 17: 192-198, 1971;
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Clinical Chemistry, Vol 17, 192-198, Copyright © 1971 by the American Association for Clinical Chemistry

A Manual and Automated Procedure for Measuring Serum Cholinesterase Activity and Identifying Enzyme Variants

Differentiation by Means of Tris and Phosphate Buffers

Philip J. Garry 1

1 Department of Pediatrics, College of Medicine, The Ohio State University and Children’s Hospital Research Foundation, 561 S. 17th. St., Columbus, Ohio 43205.

A simplified manual and automated method is described for measuring serum cholinesterase activity and identifying the homozygous "usual," heterozygous, and homozygous "atypical" forms of the enzyme. With butyrylthiocholine as substrate at pH 7.4, the activity is determined in two 0.05 mol/liter buffer systems, Tris and phosphate. The "usual" enzyme has the same activity in both buffer systems. The phosphate buffer inhibits the heterozygous enzyme about 13% and the "atypical" enzyme about 43% relative to its activity in Tris buffer. The range of normal serum cholinesterase values (measurements on plasma from 1842 preschool children) is 2.10 to 5.25 U (mean, 3.67 U). In this population, 3.28% of the Caucasians, but only 0.29% of the Negroes, were heterozygous for the trait.


Key Words: normal values for preschool children • racial differences in serum cholinesterase • "usual," heterozygous, and "atypical" forms • butyrylthiocholine substrate • AutoAnalyzer

Submitted on October 19, 1970
Accepted on January 6, 1971




The following articles in journals at HighWire Press have cited this article:


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 ScienceHome page
A. H. Lubin, P. J. Garry, and G. M. Owen
Sex and Population Differences in the Incidence of a Plasma Cholinesterase Variant
Science, July 9, 1971; 173(3992): 161 - 164.
[Abstract] [PDF]


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Copyright © 1971 by the American Association for Clinical Chemistry.