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Clinical Chemistry 17: 229-230, 1971;
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Clinical Chemistry, Vol 17, 229-230, Copyright © 1971 by the American Association for Clinical Chemistry

Modification of an Automated Procedure for Serum Cholesterol, which Permits the Quantitative Estimation of Cholesterol Esters

A. L. Siegel 1 and B. C. Bowdoin 1

1 Department of Clinical Pathology and the Clinical Research Center, University of Alabama Medical School and University Hospital, Birmingham, Ala. 35233.

Changes are described in AutoAnalyzer Methodology N-24a, for the automated determination of serum total cholesterol, that increase the reaction temperature of the mixture of sample and acid color reagent. The modified procedure eliminates the inaccuracies that attended the original methodology and allows the cholesterol ester moiety in the serum total cholesterol to be quantitated.


Key Words: AutoAnalyzer

Submitted on November 27, 1970
Accepted on December 11, 1970






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Copyright © 1971 by the American Association for Clinical Chemistry.