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Clinical Chemistry 17: 290-295, 1971;
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Clinical Chemistry, Vol 17, 290-295, Copyright © 1971 by the American Association for Clinical Chemistry

Electrophoretic Separation on Agarose Thin Film of Isoenzymes of Alkaline Phosphatase from Human Serum and Tissue

J. A. Demetriou 1 and J. M. Beattie 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

An electrophoretic method is presented for separating alkaline phosphatase isoenzymes on agarose gel by using barbital buffer. agr-Naphthyl AS-MX phosphate was converted to the highly fluorescent agr-naphthol AS-MX, to detect the zones of enzymic activity on the thin film. The fluorescent zones on the electropherograms were scanned with a recording fluorometer and the electrophoretic mobilities of the enzymes were compared with those of the plasma proteins. This method was used to assess the isoenzyme patterns in human sera and tissues (liver, bone, kidney, spleen, and placenta).


Key Words: agr-naphthyl AS-MX phosphate • fluorometry • isoenzymes in human sera and tissues, normal patterns and values

Submitted on December 9, 1970
Accepted on January 15, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.