Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 17: 307-310, 1971;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perez, V. J.
Right arrow Articles by Bahn, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perez, V. J.
Right arrow Articles by Bahn, B. A.

Clinical Chemistry, Vol 17, 307-310, Copyright © 1971 by the American Association for Clinical Chemistry

Ethanol in Brain, as Assayed by Microfluorometry

Vernon J. Perez 1, Theodore J. Cicero 1, and Barbara A. Bahn 1

1 Laboratory of Neuropsychology, Department of Psychiatry, Washington University School of Medicine, 4940 Audubon Ave., St. Louis, Mo. 63110.

Gas—liquid chromatography (GLC), the technique most often used to measure ethanol in body fluids, is not practical for determining ethanol in tissues. Previously described fluorometric assays were modified for the enzymatic measurement of ethanol in brain and, for comparison, in blood of rats 1 h after intraperitoneal injection of 2.0 g of ethanol per kg of body weight. Mean ethanol content of brain was 23.43 mmol/kg and of blood, 24.18 mmol/ liter. Accuracy of the method was verified by GLC of the blood samples, which gave a mean ethanol content of 24.29 mmol/liter. No endogenous ethanol was measurable fluorometrically or by GLC in blood or brain samples from control animals injected with physiological saline solution (9 g/liter). Because samples of only 1 µl are required, the procedure is particularly useful when ethanol is to be measured in minute brain samples or when only very small volumes of blood are available.


Key Words: ethanol in blood • rats • gas chromatography • enzymic assay

Accepted on January 19, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the American Association for Clinical Chemistry.