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Clinical Chemistry 17: 323-325, 1971;
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Clinical Chemistry, Vol 17, 323-325, Copyright © 1971 by the American Association for Clinical Chemistry

beta-Glycerophosphate and Thymolphthalein Monophosphate Compared as Substrates for Determining Alkaline Phosphatase Activity in Serum

Fram R. Dalal 1, Mohammed Akhtar 1, Kwang Hi Shin 1, and Seymour Winsten 1

1 Division of Laboratories, Albert Einstein Medical Center, York and Tabor Roads, Philadelphia, Pa. 19141.

beta-Glycerophosphate and sodium thymolphthalein monophosphate were compared clinically and methodologically as substrates for alkaline phosphatase assay. The relative simplicity of the thymolphthalein procedure, its adaptability to semi-automated and micro methods, and its apparent clinical reliability indicate that it is a better substrate for serum alkaline phosphatase assay than is beta-glycerophosphate.


Key Words: Bodansky procedure • normal and pathological ranges

Submitted on January 18, 1971
Accepted on February 2, 1971







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Copyright © 1971 by the American Association for Clinical Chemistry.