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Clinical Chemistry 17: 332-334, 1971;
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Clinical Chemistry, Vol 17, 332-334, Copyright © 1971 by the American Association for Clinical Chemistry

Effect of Triton X-100 on Electrophoretic Mobility of Red Blood Cell Ghosts from Normal Individuals and Patients With Tay—Sachs Disease

M. Pourfar 1 and S. Levy 1

1 Department of Pediatrics and Isaac Albert Research Institute, Kingsbrook Jewish Medical Center, Brooklyn, N.Y. 11203.

The negative surface charge of red blood cell ghosts and their anodal electrophoretic migration are largely due to their sialic acid moiety. The RBC of patients with Tay—Sachs disease are thought to contain increased amounts of sialic acid. Electrophoretic mobility and ganglioside content of RBC ghosts were compared in four patients with Tay—Sachs disease and eight normal controls. There was no measurable difference in electrophoretic mobility on cellulose acetate or acrylamide gel. The sialic acid content of lyophilized RBC ghosts was about the same for samples from both patients and controls. The nonionic detergent, Triton X-100, interacts with the ghost glycolipoprotein, causing a reversal of migration, both on cellulose acetate and moving-boundary electrophoresis. Acrylamide gel, by its sieving effect, disrupts the complex, which then migrates anodally again.


Key Words: moving-boundary electrophoresis • electrophoresis on cellulose acetate or acrylamide • sialic (neuraminic) acids • glycolipoprotein

Submitted on April 27, 1970
Accepted on February 5, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.