Clinical Chemistry
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Clinical Chemistry 17: 358-362, 1971;
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Clinical Chemistry, Vol 17, 358-362, Copyright © 1971 by the American Association for Clinical Chemistry

Direct Photometric Determination of Globulin in Serum

Harry Goldenberg 1 and Patricia A. Drewes 1

1 Research Department, Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

A convenient method for direct photometric determination of total globulin in serum has been developed using a one-tube, one-reagent system. The analysis is based on a variation of the Hopkins-Cole reaction, in which glyoxylic acid condenses with the tryptophan residues present in globulin to produce a purple color. In the presence of copper and under the conditions of the analysis, color intensity is proportional to serum globulin concentration. The method is standardized with either a serum control or a suitable tryptophan derivative (e.g., acetyl-DL-tryptophan). There is little or no interference from free tryptophan, albumin, bilirubin, lipemia, or mild hemolysis. The precision of the method is ±3% (95% confidence limits), and results agree with those obtained by the salt fractionation method of Wolfson et al. [Amer. J. Clin. Pathol. 18, 723 (1948)]: t = -0.88; critical t = 2.02; 95% confidence limits.


Key Words: normal values • comparison with salt fractionation method • Hopkins—Cole reaction • diagnostic aid • N-acetyl-DL-tryptophan as a reference standard • A/G ratio

Submitted on October 2, 1970
Accepted on February 8, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.