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Clinical Chemistry 17: 363-368, 1971;
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Clinical Chemistry, Vol 17, 363-368, Copyright © 1971 by the American Association for Clinical Chemistry

Biological Applications of the Carbon Rod Atomizer in Atomic Absorption Spectroscopy

1. Preliminary Studies on Magnesium, Iron, Copper, Lead, and Zinc in Blood and Plasma

Jaroslav P. Matouscaronek 1 and Brian J. Stevens 1

1 Varian Techtron Pty. Ltd., North Springvale, 3170, Victoria; and Pathology Department, Royal Children's Hospital, Parkville, 3052, Victoria, Australia.

A new technique in atomic absorption spectroscopy is described, which replaces the use of a flame. Samples are injected into a cavity drilled transversely through a carbon rod, and subsequently dried, ashed, and atomized by the application of electric current. The resulting brief absorption signal is recorded and compared with standards. The procedure is particularly suitable for biological work since sample volume requirements are in the submicroliter range. The samples require no pretreatment. The method has given excellent results initially when applied to the measurement of magnesium, iron, copper, lead, and zinc in blood and plasma.


Key Words: capillary blood samples • trace-metal determinations in serum, plasma, packed red cells • comparison with flame absorption spectrophotometry

Submitted on January 11, 1971
Accepted on February 11, 1971




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