Clinical Chemistry
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Clinical Chemistry 17: 374-377, 1971;
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Clinical Chemistry, Vol 17, 374-377, Copyright © 1971 by the American Association for Clinical Chemistry

Automated Immunoprecipitin Technique for Analysis of the Third Component of Complement (C'3) in Human Serum

L. M. Killingsworth 1, John Savory 2, and Perry O. Teague 2

1 Department of Pathology, J. Hillis Miller Health Center, University of Florida, Gainesville, Florida, 32601.
2 Department of Pathology, University of Florida, Shands Teaching Hospital, Gainesville, Fla. 32601.

An automated continuous-flow procedure has been developed for measuring C'3 in human serum, by reaction with specific antiserum. The procedure is standardized with pooled human serum, appropriate dilutions of which had previously been measured for C'3 by a reference method. C'3 values obtained by this new automated procedure correlate with values obtained by radial immunodiffusion. The precision (CV) of this method is 1.65%.


Key Words: AutoAnalyzer • radial immunodiffusion • nephelometry

Submitted on January 25, 1971
Accepted on February 18, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.