Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 17: 378-381, 1971;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coburn, S. P.
Right arrow Articles by Fuller, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coburn, S. P.
Right arrow Articles by Fuller, R. W.

Clinical Chemistry, Vol 17, 378-381, Copyright © 1971 by the American Association for Clinical Chemistry

An Improved Method for Measuring Blood Concentrations of Phenylpyruvic Acid

S. P. Coburn 1, J. D. Mahuren 1, and R. W. Fuller 1

1 Fort Wayne State Hospital and Training Center, 801 E. State Blvd., Fort Wayne, Ind. 46805 (S. P. C. and J. D. M.); and Lilly Research Laboratories, Indianapolis, Ind. 46206.

We describe an improved determination of phenylpyruvic acid (PPA) in blood plasma by ultraviolet spectrophotometric measurement of an enol— borate complex. Sensitivity and recovery are well suited for accurate analysis in the range of 1.25 to 25 mg/liter. Sera from 10 fasting phenylketonuric subjects averaged 15.3 mg/liter (range, 4.8 to 25.0 mg/liter). A pronounced diurnal rhythm in plasma PPA, with peak concentrations occurring between noon and 6 p.m., was detected in patients with untreated phenylketonuria.


Key Words: ultraviolet spectrophotometry • phenylketonuria • diurnal rhythmn in plasma PPA • hyperphenylalaninemia • enol—borate complex

Submitted on November 23, 1970
Accepted on February 18, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the American Association for Clinical Chemistry.