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Clinical Chemistry 17: 382-385, 1971;
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Clinical Chemistry, Vol 17, 382-385, Copyright © 1971 by the American Association for Clinical Chemistry

Gas Chromatographic Assay of Underivatized 5,5-Diphenylhydantoin (Dilantin) in Plasma Extracts

Dian Sampson 1, Irene Harasymiv 1, and W. J. Hensley 1

1 Department of Biochemistry, Royal Prince Alfred Hospital, Camperdown, N.S.W. 2050 Australia.

A simple, rapid, and accurate method is described for determining diphenylhydantoin concentrations in plasma. An internal standard, primidone (primaclone), is added to 2 ml of acidified plasma, and primidone and diphenylhydantoin are extracted into chloroform. The extract, washed with hexane and concentrated, is injected into a gas chromatograph. Extraction and measurement of a single sample requires about 30 min, and as many as 40 samples can be assayed in a working day. Both therapeutic and overdose concentrations of diphenylhydantoin in plasma can be detected and measured. Nine other acidic drugs, added to plasma samples, did not interfere with the analysis.


Key Words: phenobarbital identification • emergency determinations

Submitted on October 7, 1970
Accepted on February 19, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.