Clinical Chemistry
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Clinical Chemistry 17: 386-387, 1971;
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Clinical Chemistry, Vol 17, 386-387, Copyright © 1971 by the American Association for Clinical Chemistry

High-Voltage Thin-Layer Electrophoretic Method for Detecting Histidinemia

Dale A. Newton 1 and George K. Summer 1

1 Department of Biochemistry, School of Medicine, and Biological Sciences Center, Child Development Institute, University of North Carolina, Chapel Hill, N. C. 27514.

A high-voltage thin-layer electrophoretic method is described for separating urinary histidine and imidazoleacetic, imidazolelactic, and imidazolepyruvic acids. The procedure provides a reliable confirmation of the diagnosis of histidinemia.


Key Words: imidazoleacetic, -lactic, and -pyruvic acids in urine

Submitted on January 9, 1971
Accepted on February 18, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.