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Clinical Chemistry 17: 392-396, 1971;
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Clinical Chemistry, Vol 17, 392-396, Copyright © 1971 by the American Association for Clinical Chemistry

Direct Measurement of Iodine Production by Sonic Extracts of Polymorphonuclear Leukocytes

Lawrence R. DeChatelet 1, Charles E. McCall 1, and M. Robert Cooper 1

1 Departments of Biochemistry and Medicine, The Bowman Gray School of Medicine, Winston-Salem, N.C. 27103.

We describe an enzymatic reaction between iodide ion, H2O2, and neutrophil sonicates, in which free iodine is formed. Some characteristics of the reaction are: (a) it is catalyzed by sonic extracts of human neutrophils, by purified horseradish peroxidase, or purified human myeloperoxidase, but not by sonic extracts of rabbit alveolar macrophages or beef liver catalase; (b) iodine is the product, as shown by its absorption spectrum and the absorption spectrum of the starch adduct; (c) the reaction is proportional to the amount of neutrophil sonicate added, and has a pH optimum near 4.0. Reaction is not linear with respect to time, owing to denaturation of the enzyme. Kinetic data indicate that the enzyme may be allosteric with respect to iodide and is inhibited by high concentrations of H2O2. These represent possible sources of control of the reaction.


Key Words: peroxidase • bactericidal activity of the neutrophil • spectrophotometry • myeloperoxidase in alveolar macrophages • neutrophil physiology and pathophysiology myeloperoxidase deficiency

Submitted on January 18, 1971
Accepted on February 22, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.