Clinical Chemistry
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Clinical Chemistry 17: 397-399, 1971;
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Clinical Chemistry, Vol 17, 397-399, Copyright © 1971 by the American Association for Clinical Chemistry

Micromethod for Measuring Pentoses by Use of an Aniline Reagent

Jesse F. Goodwin 1

1 General Clinical Research Center for Children, Wayne State University School of Medicine, and Children's Hospital of Michigan, Detroit, Mich. 48202.

Aniline, in an acetic acid solution containing borate and thiourea, reacts with pentose sugars to yield a highly colored chromogen that absorbs maximally at 480 nm. The reagent may be used to measure pentoses in dilute protein solutions and serum without first removing protein. Bilirubin and hemoglobin do not interfere extensively with the method, but this interference is substantially decreased if a protein-free filtrate is used. An optimal procedure is presented for the measurement of pentoses in the presence of glucose, involving use of glucose oxidase and the aniline reagent. The method is easy to perform, sensitive, and reproducible.

Submitted on February 4, 1971
Accepted on March 1, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.