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Clinical Chemistry 17: 400-402, 1971;
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Clinical Chemistry, Vol 17, 400-402, Copyright © 1971 by the American Association for Clinical Chemistry

Ultramicrodetermination of Plasma Urea by Reaction with Diacetylmonoxime—Antipyrine without Deproteinization

Giovanni Ceriotti 1

1 Laboratorio Centrale di Analisi, Ospedale Civile di Padova, Padua, Italy.

Urea can be directly determined in serum or plasma by reaction with diacetylmonoxime—antipyrine: 5 µl of the sample is mixed with 5 ml of the chromogenic reagent, and the mixture is boiled 15 min, cooled, and read at 460 nm. The color is stable for 30 min in the light. The reagent is composed of (a) 0.4 g of antipyrine per 100 ml of H2SO4 (20 ml/100 ml), containing 5 mg of ferric sulfate per 100 ml: 1 (or 2) volume(s), and (b) 0.5 g of diacetylmonoxime per 100 ml of acetic acid (5 ml/100 ml): 1 volume.


Key Words: AutoAnalyzer

Submitted on November 23, 1970
Accepted on February 4, 1971




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Copyright © 1971 by the American Association for Clinical Chemistry.