Clinical Chemistry
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Clinical Chemistry 17: 417-422, 1971;
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Clinical Chemistry, Vol 17, 417-422, Copyright © 1971 by the American Association for Clinical Chemistry

An Automated, Fluorometric Procedure for Determining Serum Lipase

Martin Fleisher 1 and Morton K. Schwartz 1

1 Department of Biochemistry, Memorial Hospital for Cancer and Allied Diseases, New York, N. Y. 10021.

Currently available routine assays for lipase (glycerol ester hydrolase EC 3.1.1.3) are lengthy and laborious, and may utilize nonspecific substrates. An automated continuous-flow procedure has been developed for the fluorometric determination of serum lipase activity. This involves the use of a fluorescein fatty acid ester, monodecanoyl fluorescein (MDF), as substrate. This compound is not fluorescent, but one of its hydrolytic products, fluorescein, is intensely fluorochromatic. Under the conditions of the assay, in which 0.5 mmol of MDF is used per liter, the hydrolysis is linear with time and follows zero-order kinetics through the hydrolysis of at least 35% of the substrate. Determinations are carried out on 0.1 ml serum at the rate of 60 assays/hour. By this assay, mean activity in the serum of normal persons is 751 units (SD, 38 units).


Key Words: AutoAnalyzer • monodecanoyl fluorescein as substrate • kinetics • normal values • fluorometry

Submitted on February 19, 1971
Accepted on March 15, 1971




The following articles in journals at HighWire Press have cited this article:


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L. J. Lifton, K. A. Slickers, D. A. Pragay, and L. A. Katz
Pancreatitis and Lipase: A Reevaluation With a Five-Minute Turbidimetric Lipase Determination
JAMA, July 1, 1974; 229(1): 47 - 50.
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Copyright © 1971 by the American Association for Clinical Chemistry.