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Clinical Chemistry 17: 423-426, 1971;
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Clinical Chemistry, Vol 17, 423-426, Copyright © 1971 by the American Association for Clinical Chemistry

Macroamylasemia: Rapid Detection Method

Louis Fridhandler 1, J. Edward Berk 1, and Masatoshi Ueda 1

1 Department of Medicine, University of California, Irvine, Calif. 92664.

A reliable method is presented for rapidly screening human sera to detect macroamylasemia. A mixture of the serum to be tested, Blue Dextran, and cytochrome c is filtered through a small column of cross-linked dextran gel (Sephadex G-100). Amylase in the effluent fractions is detected by incubation with amylose and subsequent addition of iodine. Macroamylase, if present, is found in the blue-colored fractions, while amylase of normal molecular weight appears in the fractions colored by cytochrome c.


Key Words: gel filtration • cytochrome c and Dextran Blue as markers • diagnostic screening • hyperamylasemia

Submitted on February 15, 1971
Accepted on March 16, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.