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Clinical Chemistry 17: 438-439, 1971;
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Clinical Chemistry, Vol 17, 438-439, Copyright © 1971 by the American Association for Clinical Chemistry

Simple Assay for Urinary Estrogens in Nonpregnancy

T. M. Chu 1, Yoshio Osawa 1, and Gustavo Reynoso 1

1 Roswell Park Memorial Institute (T. M. C., G. R.) and Medical Foundation of Buffalo (Y. O.), Buffalo, New York, 14203.

A simple procedure is described for the selective extraction of urinary estrogens by concentration on XAD-2 resin and elution with ethanol:water (30:70, by vol). Fractionation of estrogens, with high sensitivity and good recovery, from urine of nonpregnant individuals is made possible by combined use of XAD-2 resin extraction and gas—liquid chromatography.


Key Words: gas chromatography

Submitted on January 27, 1971
Accepted on March 6, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.