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Clinical Chemistry 17: 440-441, 1971;
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Clinical Chemistry, Vol 17, 440-441, Copyright © 1971 by the American Association for Clinical Chemistry

Blood Glucose Determination without Deproteinization, with Use of o-Toluidine in Dilute Acetic Acid

Giovanni Ceriotti 1

1 Laboratorio Centrale di Analisi, Ospedale Civile di Padova (Italy).

A method is described for determining glucose in 25 µl of plasma. o-Toluidine is used in weak acetic acid solution. Addition of the emulsifier "Cremophor" obviates the need for deproteinization. The reaction is complete after 15 min at 100°C, and the color is stable for longer than 24 h. The blank is almost colorless. The reagent is stable, colorless, not viscous, and convenient to handle.

Submitted on November 23, 1970
Accepted on March 9, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.