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Clinical Chemistry 17: 486-491, 1971;
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Clinical Chemistry, Vol 17, 486-491, Copyright © 1971 by the American Association for Clinical Chemistry

Modified Fluorometric Method for Determining Plasma Catecholamines

Joseph D. Sapira 1, Thelma Klaniecki 1, and Margaret Rizk 1

1 Psychosomatic and Clinical Pharmacology Sections, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pa. 15212.

Plasma catecholamines were fluorometrically determined by a modification of the standard alumina-trihydroxyindole method in which radioactive tracer catecholamines are used to follow the chromatographic behavior of the endogenous catecholamines on alumina. This modification permits appropriate fluorometry of that portion of the eluate with the highest concentration of catecholamines, because incomplete elution of the total endogenous catecholamines can be corrected by use of the isotope recovery data. Additionally, a low column blank was obtained; this method of blank preparation may have obviated some of the known difficulties associated with unoxidized and incomplete plasma blanks that have traditionally been used.


Key Words: alumina—trihydroxyindole method • radioactive tracer catecholamines • column chromatography

Submitted on February 11, 1971
Accepted on March 18, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.