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Clinical Chemistry 17: 492-494, 1971;
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Clinical Chemistry, Vol 17, 492-494, Copyright © 1971 by the American Association for Clinical Chemistry

A More Sensitive Spectrophotometric Method for Determination of Isoniazid in Serum or Plasma

Leslie Eidus 1 and Anita M. T. Harnanansingh 1

1 National Reference Centre for Tuberculosis, Canadian Communicable Disease Centre, Department of National Health and Welfare, Ottawa 3, Canada.

A sensitive spectrophotometric method is described for determining isoniazid in serum or plasma. It is extracted from biological fluids by a mixture of organic solvents and re-extracted into hydrochloric acid. In this medium, isoniazid reacts with trans-cinnamaldehyde to form a derivative absorbing maximally at 340 nm. The method is five times more sensitive than published procedures in which vanillin or piperonal is used as coupling reagent for the estimation of isoniazid.


Key Words: extraction with organic solvents • trans-cinnamaldehyde reagent

Submitted on February 11, 1971
Accepted on March 23, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.