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Clinical Chemistry 17: 501-504, 1971;
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Clinical Chemistry, Vol 17, 501-504, Copyright © 1971 by the American Association for Clinical Chemistry

Rapid Method for Protein Quantitation by Electroimmunodiffusion

Charles W. Gill 1, Craig L. Fischer 1, and Carolinda L. Holleman 1

1 Brown & Root-Northrop, Lunar Receiving Laboratory (C.W.G. and C.L.H.), and Division of Preventive Medicine (C.L.F.), National Aeronautics & Space Administration, Manned Spacecraft Center, Houston, Texas 77058.

In the electroimmunodiffusion method described, a Mylar-backed cellulose acetate membrane plate is used as a support medium. Results are obtained faster, less preparation time is required, and the method is less expensive because less antisera is needed. Results with the method are reproducible (CV, 5%), correlate well with results by the single radial immunodiffusion method, and sensitivity is similar to the electroimmunodiffusion method in which agar gel is used as a support medium.


Key Words: Mylar-backed cellulose acetate plate • radial immunodiffusion

Submitted on January 28, 1971
Accepted on March 29, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.