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Clinical Chemistry 17: 505-511, 1971;
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Clinical Chemistry, Vol 17, 505-511, Copyright © 1971 by the American Association for Clinical Chemistry

Method for Quantitative Measurement of Xanthurenic Acid in Urine

Mary Jean Wallace 1, Henry W. Vaillant 1, and Hilton A. Salhanick 1

1 Center for Population Studies, Harvard University, 665 Huntington Ave., Boston, Mass. 02115.

A method is reported for determining urinary xanthenuric acid (XA), a metabolite of tryptophan. XA is extracted from urine with isobutanol (2-methyl-1-propanol), transferred to ammonium hydroxide, and separated from other urinary metabolites on thin-layer cellulose plates with use of 0.2 acetate buffer, mol/liter, pH 5.4. The XA is measured spectrophotometrically after diazotization with 2-chloro-4-nitroaniline. The method, which is specific for XA, can be used to measure amounts as low as 0.4 µg/ml of urine. It has a range from 0 to 24 µg/ml, and results within this range are reproducible within approximately ±5%. Data obtained by this procedure are comparable to results reported for other methods.


Key Words: thin-layer chromatography • spectrophotometry • diazotization with 2-chloro-4-nitroaniline • tryptophan metabolism • vitamin B6 deficiency

Submitted on October 29, 1970
Accepted on March 27, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.