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Clinical Chemistry 17: 525-528, 1971;
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Clinical Chemistry, Vol 17, 525-528, Copyright © 1971 by the American Association for Clinical Chemistry

A Simplified Assay for Porphyrins in Whole Blood

Samuel R. Heller 1, Robert F. Labbe 1, and Janet Nutter 1

1 Fircrest Research Laboratories, Department of Pediatrics, University of Washington, Seattle, Wash. 98105.

Recent investigations concerning the significance of porphyrins in erythrocytes indicate a need for a rapid quantitative assay, easily adaptable to routine clinical use. To fill this need, we have developed a simplified procedure for the measurement of porphyrins (essentially protoporphyrin) in whole blood. Blood is stirred with acetone—ethyl acetate and the porphyrins are extracted with formic acid—ether. The porphyrins are then extracted into HCl and measured spectrophotometrically at three wavelengths, a procedure that allows a corrected absorbance to be calculated, to minimize error caused by interfering substances. The assay requires little apparatus, is simple and dependable, and the results correlate well with those obtained by use of a more complex conventional procedure.


Key Words: protoporphyrin • spectrophotometry • diagnosis of hematologic disorders • porphyrias • normal values • nutritional iron status

Submitted on February 16, 1971
Accepted on March 30, 1971




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