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Clinical Chemistry, Vol 17, 535-537, Copyright © 1971 by the American Association for Clinical Chemistry
1 Kings Park Laboratory, 10 King’s Park Road, Perth,
Western Australia, 6005.
A colorimetric adaptation of the fluorometric method of Noble and Campbell is described. Decreased sensitivity, caused by substituting colorimetry for fluorometry, has been improved by increasing the proportion of serum to isopropanol extraction fluid from 1 in 21 to 1 in 11, and by placing an adjustable range expander between colorimeter and recorder. This accurate and reliable adaptation has obviated the need for an expensive fluorometer.
Submitted on March 29, 1971
Accepted on April 16, 1971
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