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Clinical Chemistry 17: 548-550, 1971;
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Clinical Chemistry, Vol 17, 548-550, Copyright © 1971 by the American Association for Clinical Chemistry

Single Stable Reagent for Creatine Kinase Assay

Charles Bishop 1, T. M. Chu 1, and Z. K. Shihabi 1

1 Biochemistry Laboratory, Buffalo General Hospital, Buffalo, N. Y. 14203, and the Department of Medicine, State University of New York at Buffalo.

A single stable reagent for spectrophotometric assay of creatine kinase in one step is described. Reagent cost has been decreased by running the reaction at Km conditions for creatine phosphate and relating the activity to maximum velocity.


Key Words: Cleland’s reagent • 2-aminoethylisothiouronium • mercaptoethanol • mercaptoethanolamine • cysteine • normal range • spectrophotometry

Submitted on March 10, 1971
Accepted on April 2, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.