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Clinical Chemistry 17: 581-584, 1971;
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Clinical Chemistry, Vol 17, 581-584, Copyright © 1971 by the American Association for Clinical Chemistry

Antibodies to Testosterone-3-Bovine Serum Albumin, Applied to Assay of Serum 17beta-ol Androgens

Jeffrey C. Chen 1, Elinor M. Zorn 1, Marvin C. Hallberg 1, and Ralph G. Wieland 1

1 Department of Endocrinology and Metabolism, Division of Medicine, Saint Luke’s Hospital, Cleveland, Ohio 44104.

Testosterone was conjugated to bovine serum albumin at carbon 3, and antibodies to this conjugate were developed in virgin female rabbits. This antiserum, diluted 1:3200, was used to measure testosterone. With tritiated testosterone and anti-rabbit ggr-globulin as the second antibody, a doseresponse curve was obtained for doses in the range 0.1-10 ng. The antibody cross-reacts extensively with dihydrotestosterone, so that dihydrotestosterone is measured with testosterone in extracted but unchromatographed serum. Results for pooled serum from men, as measured repeatedly by radioimmunoassay and competitive binding assay for testosterone, agreed well. Seventeen normal men had serum 17beta-ol androgen concentrations of 667 ng/100 ml (SD, ±248); seventeen normal, menstruating women had a mean concentration of 69 ng/100 ml (SD, ±18). Values for 17beta-ol androgens in adolescent males agreed reasonably with testosterone values reported by other investigators. Values are presented for patients with various androgenic disorders. This procedure is easy; many samples can be processed at one time.


Key Words: normal values for men, women, boys • values during androgenic disorders • testosterone • dihydrotestosterone

Submitted on February 26, 1971
Accepted on April 2, 1971




The following articles in journals at HighWire Press have cited this article:


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Measurement of Free Testosterone in Normal Women and Women with Androgen Deficiency: Comparison of Methods
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 525 - 533.
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C. Wang, D. H. Catlin, L. M. Demers, B. Starcevic, and R. S. Swerdloff
Measurement of Total Serum Testosterone in Adult Men: Comparison of Current Laboratory Methods Versus Liquid Chromatography-Tandem Mass Spectrometry
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 534 - 543.
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Copyright © 1971 by the American Association for Clinical Chemistry.