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Clinical Chemistry 17: 595-601, 1971;
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Clinical Chemistry, Vol 17, 595-601, Copyright © 1971 by the American Association for Clinical Chemistry

Hepatitis-Associated Antigen (HAA). I. Quantitation by Electrophoresis in Antibody-Containing Agarose Gel by Use of a Primary Standard of Purified HAA

Thomas Boenisch 1 and Gerson Katz 1

1 Leary Laboratory, Inc., Immunochemistry Department, 43 Bay State Road, Boston, Mass. 02215.

Hepatitis-associated antigen (HAA) has been isolated from pooled HAA positive human serum for use as a purified HAA primary standard and in production of antiserum in guinea pigs. These reagents were used in conjunction with electrophoresis in antibody-containing agarose gel, to simultaneously screen for and measure HAA. The smallest concentration of antigen that we could detect was 2 mg/100 ml of serum; the highest concentration of antigen encountered was 170 mg/100 ml. The method was compared with the Ouchterlony double diffusion and counterelectrophoresis techniques. Several antisera against HAA are compared with each other and their titers expressed in milligrams of HAA precipitated per milliliter of antiserum.


Key Words: Australia antigen • immunoelectrophoresis • double diffusion (Ouchterlony) technique • counterelectrophoresis • immunoelectroosmophoresis • diagnosis of acute viral hepatitis

Submitted on April 6, 1971
Accepted on April 27, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.