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Clinical Chemistry 17: 614-621, 1971;
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Clinical Chemistry, Vol 17, 614-621, Copyright © 1971 by the American Association for Clinical Chemistry

Increased AutoAnalyzer Dialysis of Calcium and Magnesium in Presence of Protein

John A. Lott 1 and Thomas S. Herman 1

1 Department of Pathology, Ohio State University, 410 West 10th Ave., Columbus, Ohio 43210.

We have studied calcium and magnesium determination with the AutoAnalyzer, using dialysis, and measurement by atomic absorption spectrophotometry. The fraction of calcium and magnesium that dialyzes is proportional to the protein content of the sample; therefore an error is introduced when aqueous solutions of calcium or magnesium salts are used as standards. Apparently, the presence of proteins causes increased dialysis of Ca+2 and Mg+2.


Key Words: serum calcium and magnesium analysis • dialysis • analytical error • normal values • Donnan membrane effect • AutoAnalyzer

Submitted on October 7, 1970
Accepted on May 4, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.