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Clinical Chemistry 17: 707-714, 1971;
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Clinical Chemistry, Vol 17, 707-714, Copyright © 1971 by the American Association for Clinical Chemistry

A Systems Analysis of GEMSAEC Precision Used as a Kinetic Enzyme Analyzer

Ernest Maclin 1

1 Electro-Nucleonics, Inc., Fairfield, N. J. 07006.

Use of the GEMSAEC as a kinetic enzyme analyzer has been one of the most promising applications for this instrument. Several important variables must be defined if a practical system and components are to be developed for use in the clinical laboratory. The effect and inter-relationship of four of these are defined and analyzed to determine their impact on the precision of GEMSAEC: starting absorbance of the sample—reagent mixture, rate of change of absorbance, length of time for which the linear kinetic reaction progresses, and the uncertainty of the analog-to-digital conversion combined with electronic noise. A set of curves is generated from equations derived to demonstrate the coefficient of variation based on these variables. The influence of these variables, as compared to the variations of sample and reagent addition and of temperature control, is also briefly reviewed. The performance of the ENI GEMSAEC against these errors is summarized. The techniques described also define a convenient and practical way to evaluate the limits of precision that can be expected from analyzers for kinetic analysis based on the rate of the reaction rather than on a specific chemical determination.


Key Words: spectrophotometric error analyses • digitization effects on spectrophotometric instruments • the effect of chemical and instrument parameters on precision of rate reactions • Rotoloader • instrumental variables • precision limits for fast analyzers






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Copyright © 1971 by the American Association for Clinical Chemistry.