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Clinical Chemistry 17: 774-781, 1971;
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Clinical Chemistry, Vol 17, 774-781, Copyright © 1971 by the American Association for Clinical Chemistry

High-Resolution Chromatography of Complex Organic Mixtures

Robert L. Stevenson 1 and Carl A. Burtis 1

1 Varian Aerograph, 2700 Mitchell Drive, Walnut Creek, Calif. 94598 (R. L. S.), and Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830 (C. A. B.).

The high-resolution uv analyzer has been used to investigate the effect of diet on urine profile. In addition, new applications in the food and beverage industry appear promising. More than 100 compounds, acidic to basic, can be separated. The clinical analyst may be interested in scanning a wide variety of compounds or in rapid separation of only a few compounds. For the latter purpose, the effect of changing the gradient elution program has been investigated, and conditions have been defined for rapid analysis of specific regions of the chromatogram. Additionally, the chromatogram may be simplified by fractionation—for example, by solvent extraction—before high-resolution analysis. The sensitivity of the instrument is often limited by baseline drift caused by absorbance of the buffer. Various methods for compensating for this drift have been investigated. Reproducibility of retention time, response, and computerized data processing are discussed.


Key Words: fractionation of urine, vanilla, wine • dietary effects • speeded analyses • prechromatography fractionation of urine • baseline drift and compensation • gradient elution






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Copyright © 1971 by the American Association for Clinical Chemistry.