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Clinical Chemistry 17: 891-895, 1971;
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Clinical Chemistry, Vol 17, 891-895, Copyright © 1971 by the American Association for Clinical Chemistry

Manual and Automated Methods for Urea Nitrogen Measurement in Whole Serum

Donald R. Wybenga 1, John Di Giorgio 1, and Vincent J. Pileggi 1

1 Bio-Science Laboratories, Research Department, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

A simple method is described for directly determining serum urea nitrogen, wtihout deproteinization. The urea in 20 µl of serum or plasma is reacted with diacetylmonoxime in the presence of thiosemicarbazide and cadmium ion under acid conditions. The absorbance of the resulting rose-purple solution is measured at 540 nm. Results agreed excellently with those obtained by a reference method that makes use of urease and the Berthelot phenate—hypochlorite reaction for ammonia. An automated adaptation utilizing the AutoAnalyzer is also described, which eliminates the need for a dialyzer. A further advantage is that the same reagents are used for both methods.


Key Words: rases diacetylmonoxime—thiosemicarbazide reaction • colorimetry • Cd as a color stabilizer • AutoAnalyzer • Berthelot reaction

Submitted on March 23, 1971
Accepted on May 26, 1971




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