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Clinical Chemistry 17: 903-907, 1971;
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Clinical Chemistry, Vol 17, 903-907, Copyright © 1971 by the American Association for Clinical Chemistry

Paper-Chromatographic Quantitation of 4-Hydroxy-3-methoxymandelic Acid (VMA) in Urine

Ardeshir Vahidi 1, Henry R. Roberts 1, Joseph San Filippo Jr. 1, and D. V. Siva Sankar 1

1 Squibb Institute for Medical Research, New Brunswick, N. J. 08903 (A. V. and H. R. R.); and the Department of Chemistry, Long Island University, New York and Biochemical Research Laboratory, Creedmoor Children’s Hospital, Queens Village, N. Y. 11427 (J. S. F. and D. V. S. S.).

We describe a quantitative paper-chromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p-nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of replicates of standard and sample solutions on the same chromatogram exceeded 95%.


Key Words: paper chromatography • colorimetry • diazotized p-nitroaniline color reagent • rats

Submitted on April 13, 1971
Accepted on June 3, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.