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Clinical Chemistry 17: 908-914, 1971;
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Clinical Chemistry, Vol 17, 908-914, Copyright © 1971 by the American Association for Clinical Chemistry

Coulometric Titration of Blood Glutathione

Jack H. Ladenson 1 and William C. Purdy 1

1 Department of Chemistry, University of Maryland, College Park, Md. 20742.

Conditions are described for coulometric titration of reduced glutathione with silver(I), in an ammoniacal medium. pH and buffer capacity of the medium are important variables. Metaphosphoric acid, 50 g/liter, is superior to metaphosphoric acid, 10 g/liter, or sulfosalicylic acid, 25 g/liter, for precipitating blood proteins. Results of the coulometric method are about the same as those obtained with spectrophotometric procedures in which alloxan or 5,5'-dithio-bis-(2-nitrobenzoic acid) is used.


Key Words: biamperometric end-point detection (pokirograph) • EDTA as preservative for GSH • pH and buffer capacity effects • inter-method comparison • "sulfosalicylic acid effect"

Submitted on April 24, 1971
Accepted on June 7, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.