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Clinical Chemistry 17: 921-925, 1971;
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Clinical Chemistry, Vol 17, 921-925, Copyright © 1971 by the American Association for Clinical Chemistry

A Single-Reagent Manual Method for Directly Determining Urea Nitrogen in Serum

Lowell B. Foster 1 and Jane M. Hochholzer 1

1 Medical Laboratory Associates, 1025 S. 18th St., Birmingham, Ala. 35205.

A direct method is described, in which a single reagent is used without deproteinization, for measuring blood urea nitrogen (BUN) in serum. The reagent is a mixture of diacetylmonoxime, antipyrine (phenazone), and arsenic pentoxide in acid solution. Lipemia, bilirubin to 25 mg/100 ml, hemoglobin to lg/100 ml, and protein to 20g/100 ml do not affect the results. Beer’s law is followed at 460 nm for BUN concentrations as great as 100 mg/100 ml. The direct method compares favorably with a manual urease— Berthelot color method and with the diacetylmonoxime procedure as automated for the Technicon SMA 6/60. The method (CV, 6.7%) can be completed in less than 20 mm. The reagent is stable for at least two months.


Key Words: comparison with urease BUN method • comparison with SMA 6/60 BUN method • diacetylmonoxime BUN reagent • direct BUN method • colorimetry • AutoAnalyzer

Submitted on May 31, 1971
Accepted on June 10, 1971




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Copyright © 1971 by the American Association for Clinical Chemistry.