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Clinical Chemistry 17: 931-935, 1971;
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Clinical Chemistry, Vol 17, 931-935, Copyright © 1971 by the American Association for Clinical Chemistry

A Simplified Radioimmunoassay for Human Pituitary Luteinizing Hormone in Serum

Robert A. Levine 1, Richard K. Donabedian 1, and Luis G. Sobrinho 1

1 Department of Laboratory Medicine, and Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Conn. 06510.

A simplified radioimmunoassay is described for human pituitary luteinizing hormone (LH). The principal modification over existing methods is that talc is used to separate antibody-bound hormone from free hormone by adsorption of the latter. Serum concentrations of LH in normal males, ovulatory females (follicular phase), postmenopausal females, patients with primary gonadal failure, and patients with hypopituitarism averaged 4.7, 4.6, 26.4, 30.8, and 1.9 µg of reference std. LER 907 per 100 ml of serum, respectively (10.3, 10.1, 57.8, 67.5, 4.2 milli-int. units/ml, respectively). These values are consistent with the clinical diagnoses.


Key Words: free and bound hormone • diagnostic aid • radiolabeled LH • general applicability to peptide hormones • normal and pathological values

Submitted on April 29, 1971
Accepted on June 14, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.