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Clinical Chemistry 17: 936-940, 1971;
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Clinical Chemistry, Vol 17, 936-940, Copyright © 1971 by the American Association for Clinical Chemistry

Automated Immunochemical Procedures for Measurement of Immunoglobulins IgG, IgA, and IgM in Human Serum

L. M. Killingsworth 1 and John Savory 1

1 Department of Pathology, College of Medicine, University of Florida, Gainesville, Fla. 32601.

Automated continuous-flow procedures have been developed for microdetermination of immunoglobulins IgG, IgA, and IgM in human serum. The three immunoglobulins are measured by reactions with specific commercial antiserum, and standardized by use of pooled human serum containing known concentrations of IgG, IgA, and IgM. Values determined for the immunoglobulins by the automated procedures correlate with values obtained by radial immunodiffusion. The precision of replicate determinations for the IgG, IgA, and IgM methods is 3.64, 5.11, and 6.00% (CV), respectively.


Key Words: nephelometry • AutoAnalyzer • radial immunodiffusion, correlation

Submitted on May 17, 1971
Accepted on June 15, 1971




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Copyright © 1971 by the American Association for Clinical Chemistry.