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Clinical Chemistry 17: 950-953, 1971;
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Clinical Chemistry, Vol 17, 950-953, Copyright © 1971 by the American Association for Clinical Chemistry

An AutoAnalyzer Procedure for Serum Iron and Total Iron-Binding Capacity, with Use of Ferrozine

Hugh Y. Yee 1 and Anastasia Zin 1

1 Department of Pathology, Hutzel Hospital, 432 E. Hancock Ave., Detroit, Mich. 48201.

An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine ("ferrozine"), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total iron-binding capacity is also described.

Submitted on January 29, 1971
Accepted on June 9, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.