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Clinical Chemistry 18: 37-42, 1972;
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Clinical Chemistry, Vol 18, 37-42, Copyright © 1972 by the American Association for Clinical Chemistry

Manual and Automated Determination of Immunoglobulins in Unconcentrated Cerebrospinal Fluid

J. Savory 1, M. G. Heintges 1, L. M. Killingsworth 1, and J. M. Potter 1

1 Department of Pathology, University of Florida College of Medicine and The Shands Teaching Hospital, University of Florida, Gainesville, Fla. 32601.

Manual procedures have been developed for determination in human cerebrospinal fluid of the immunoglobulins IgG, IgA, and IgM. An automated continuous-flow procedure for determination of IgG in human CSF is also described. The immunoglobulins are quantitated in both cases by immunoprecipitin reactions, with use of commercial antiserum. Values determined for the immunoglobulins by the manual method correlate with values for gamma globulin obtained by electrophoresis. The values for IgG from the manual method also correlate with IgG values determined by the automated procedure. The precision of duplicate determinations with the manual procedures for IgG, IgA, and IgM is 3.4, 1.8, and 1.0% (relative standard deviation), respectively, and that for lgG with the automated system is 3.48%.


Key Words: neurologic disease and immunoglobulins • AutoAnalyzer • Oudin technique • nephelometry

Submitted on August 9, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.