Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 52-56, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyada, D. S.
Right arrow Articles by Nakamura, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyada, D. S.
Right arrow Articles by Nakamura, R. M.

Clinical Chemistry, Vol 18, 52-56, Copyright © 1972 by the American Association for Clinical Chemistry

Albumin Quantitation by Dye Binding and Salt Fractionation Techniques

Don S. Miyada 1, Vernon Baysinger 1, Solomon Notrica 1, and R. M. Nakamura 1

1 Orange County Medical Center, Orange, Calif. 92668 (D. S. M., V. B., R. M. N.), University of California at Irvine, Irvine, Calif. (D. S. M., R. M. N.), and St. Johns Hospital, Santa Monica (S. N., presently with Laboratory Procedures, Inc., Woodland Hills, Calif.).

Two dye-binding reagents, 2-(4'-hydroxybenzeneazo)benzoic acid (HABA) and bromcresol green (BCG), for the quantitation of serum albumin have been compared with the biuret colorimetric procedure after salt fractionation. The linear regression equations for the biuret-BCG and the biuret-HABA comparisons were y = 0.43 + 0.91x and y = -0.45 + 1.15x, respectively. Their corresponding correlation coefficients were 0.93 and 0.83, respectively. The effects of icterus, lipemia, and hemolysis on the dye-binding procedures were generally greater in the case of the HABA procedure. A comparison of results with BCG and HABA for 20 sera having A/G ratios of less than 1, indicates them to be equally reliable.


Key Words: HABA method • bromcresol green method • effects of icterus, lipemia, hemolysis

Submitted on December 4, 1970






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.